Hotspot

trnN-GUU-rpl32

IRb · 110135-110622 · 488 bp

Back to Phragmipedium

Pi 0.0492

MarkerSeek score 74.90

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 488
variable_sites: 47
pi: 0.0492
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AAGCGAGCCCTTTCATTCAA	TTTGACATAGAAGTACGTTTTT	45.0	27.3	58.1	52.0	640-760 bp	1.0	49.5
trnN-GUU-rpl32_p2	AAAGCGAGCCCTTTCATTCA	TTTGACATAGAAGTACGTTTTT	45.0	27.3	58.1	52.0	641-761 bp	1.0	49.4
trnN-GUU-rpl32_p3	AAAGCGAGCCCTTTCATTCA	TTTTGACATAGAAGTACGTTTT	45.0	27.3	58.1	52.0	642-762 bp	1.0	49.4
trnN-GUU-rpl32_p4	AAGCGAGCCCTTTCATTCAA	TTTTGACATAGAAGTACGTTTT	45.0	27.3	58.1	52.0	641-761 bp	1.0	49.4
trnN-GUU-rpl32_p5	AAGCGAGCCCTTTCATTCAA	TTTTTGACATAGAAGTACGTTT	45.0	27.3	58.1	52.0	642-762 bp	1.0	49.4