Hotspot

rps12-trnV-GAC

IRb · 99535-101770 · 2236 bp

Back to Phragmipedium

Pi 0.0244

MarkerSeek score 67.40

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 2236
variable_sites: 103
pi: 0.0244
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	45.0	50.0	57.7	57.8	1973-2429 bp	1.0	84.2
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	45.0	50.0	57.7	57.8	1977-2433 bp	1.0	84.1
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	45.0	50.0	57.7	57.8	1972-2428 bp	1.0	84.1
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	45.0	55.0	57.7	58.3	1975-2431 bp	1.0	83.2
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	45.0	55.0	57.7	57.7	1958-2414 bp	1.0	82.7