Hotspot

rps3-rpl22

LSC · 85368-85498 · 131 bp

Back to Phragmipedium

Pi 0.121

MarkerSeek score 76.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 26
pi: 0.121
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGCTCAGGAGAATGGTGAGA	CAGATTCGTGGGCGTTCTTA	50.0	50.0	58.1	58.0	1115-1195 bp	1.0	87.2
rps3-rpl22_p2	CGCCCTGCAATTTGTACTTG	CAGATTCGTGGGCGTTCTTA	50.0	50.0	58.0	58.0	918-998 bp	0.6	70.5
rps3-rpl22_p3	CGCCCTGCAATTTGTACTTG	TTCGTGGGCGTTCTTATGAG	50.0	50.0	58.0	58.0	930-994 bp	0.4	57.1
rps3-rpl22_p4	TGCTCAGGAGAATGGTGAGA	TTCGTGGGCGTTCTTATGAG	50.0	50.0	58.1	58.0	1127-1191 bp	0.4	56.7
rps3-rpl22_p5	ACGTGCAATCTCTTTTCCGT	TTCGTGGGCGTTCTTATGAG	45.0	50.0	58.1	58.0	955-1019 bp	0.4	56.2