Hotspot

trnT-UGU-trnL-UAA

LSC · 47300-48372 · 1073 bp

Back to Phragmipedium

Pi 0.0413

MarkerSeek score 78.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 75
pi: 0.0413
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1123-1448 bp	1.0	87.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1131-1456 bp	1.0	87.4
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	1146-1471 bp	1.0	86.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1137-1462 bp	1.0	86.0
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	1143-1468 bp	1.0	85.6