Hotspot

petN-psbM

LSC · 29711-30760 · 1050 bp

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Pi 0.066

MarkerSeek score 79.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 88
pi: 0.066
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.58
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	737-1219 bp	1.0	89.8
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	680-1162 bp	1.0	88.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	732-1214 bp	1.0	88.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	733-1215 bp	1.0	87.5
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.5	57.9	680-1162 bp	1.0	87.5