Hotspot

atpF-atpH

LSC · 13754-14043 · 290 bp

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Pi 0.0559

MarkerSeek score 80.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 36
pi: 0.0559
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCACGGAAACAAAAGAATCGG	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	370-475 bp	1.0	81.8
atpF-atpH_p2	CCCACGGAAACAAAAGAATCG	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	371-476 bp	1.0	81.8
atpF-atpH_p3	CCACGGAAACAAAAGAATCGG	GGTCGTGGCATTAGCTCTTT	47.6	50.0	58.1	58.3	359-464 bp	1.0	80.2
atpF-atpH_p4	CCCACGGAAACAAAAGAATCG	GGTCGTGGCATTAGCTCTTT	47.6	50.0	58.1	58.3	360-465 bp	1.0	80.1
atpF-atpH_p5	CACGGAAACAAAAGAATCGGT	ATTTATGGACTGGTCGTGGC	42.9	50.0	57.4	58.0	369-474 bp	1.0	77.4