Hotspot

trnS-GCU-trnG-UCC

LSC · 8564-9391 · 828 bp

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Pi 0.0471
MarkerSeek score 76.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
828
variable_sites
64
pi
0.0471
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.55
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 501-1101 bp 1.0 62.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 500-1100 bp 1.0 62.8
trnS-GCU-trnG-UCC_p3 CTTTAGTCCACTCAGCCACC ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 491-1091 bp 1.0 62.2
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 504-1104 bp 1.0 61.7
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 503-1103 bp 1.0 61.7