Hotspot

ndhF-rpl32

SSC · 116615-117725 · 1111 bp

Back to Photinia

Pi 0.0199
MarkerSeek score 71.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1111
variable_sites
96
pi
0.0199
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.96
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTT 40.0 40.0 57.0 55.7 80-1254 bp 1.0 64.1
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT TTGAAATAGAGGTGCGCTTT 40.0 40.0 57.0 55.7 80-1255 bp 1.0 64.1
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT TTTGAAATAGAGGTGCGCTT 40.0 40.0 57.0 55.7 80-1256 bp 1.0 64.1
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT TTTTGAAATAGAGGTGCGCT 40.0 40.0 57.0 55.7 80-1257 bp 1.0 64.1
ndhF-rpl32_p5 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTTT 40.0 38.1 57.0 56.4 80-1254 bp 1.0 62.4