Hotspot

psaJ-rpl33

LSC · 70709-71182 · 474 bp

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Pi 0.0085

MarkerSeek score 60.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 28
pi: 0.0085
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	80-594 bp	1.0	78.2
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	80-639 bp	1.0	77.9
psaJ-rpl33_p3	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	80-621 bp	1.0	77.8
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	80-666 bp	1.0	77.6
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	80-664 bp	1.0	77.6