Hotspot

accD-psaI

LSC · 62193-62880 · 688 bp

Back to Photinia

Pi 0.00712

MarkerSeek score 61.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 26
pi: 0.00712
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	80-1007 bp	1.0	83.3
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.4	58.0	80-964 bp	1.0	83.3
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	80-998 bp	1.0	83.2
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	50.0	55.0	58.4	58.1	80-960 bp	1.0	83.1
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	50.0	50.0	58.4	58.1	80-971 bp	1.0	83.0