Hotspot

psbZ-trnG-GCC

LSC · 39010-39419 · 410 bp

Back to Photinia

Pi 0.0229

MarkerSeek score 61.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 43
pi: 0.0229
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTTTCTGGCTCGGCTATCC	CCGGAAGACCAAGAGCTAAC	50.0	55.0	58.0	58.0	80-1835 bp	1.0	87.3
psbZ-trnG-GCC_p2	TTTTTCTGGCTCGGCTATCC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	80-1848 bp	1.0	87.3
psbZ-trnG-GCC_p3	CTTAGCCGAGCCATTTCCTT	CCGGAAGACCAAGAGCTAAC	50.0	55.0	58.0	58.0	80-1813 bp	1.0	87.3
psbZ-trnG-GCC_p4	TTTTTCTGGCTCGGCTATCC	AGGGGCCTCTTTACCATTCT	50.0	50.0	58.0	58.0	80-1677 bp	0.888889	82.9
psbZ-trnG-GCC_p5	CTTAGCCGAGCCATTTCCTT	AGGGGCCTCTTTACCATTCT	50.0	50.0	58.0	58.0	80-1655 bp	0.888889	82.9