Hotspot

trnT-GGU-psbD

LSC · 34150-35734 · 1585 bp

Back to Photinia

Pi 0.0294
MarkerSeek score 76.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1585
variable_sites
167
pi
0.0294
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 80-1891 bp 1.0 91.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 80-1894 bp 1.0 91.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 80-1893 bp 1.0 91.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 80-1881 bp 1.0 91.1
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 80-1896 bp 1.0 91.0