Hotspot

psbA

LSC · 143-1204 · 1062 bp

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Pi 0.00167

MarkerSeek score 35.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCCTTCAACGATTCATATACACT	GTGTGCTTGGGAGTTCTTGA	34.8	50.0	55.2	58.0	1142 bp	1.0	40.4
psbA_p2	TCCTTCAACGATTCATATACAC	GTGTGCTTGGGAGTTCTTGA	36.4	50.0	53.8	58.0	1142 bp	1.0	40.4
psbA_p3	CCTTCAACGATTCATATACACT	GTGTGCTTGGGAGTTCTTGA	36.4	50.0	53.8	58.0	1141 bp	1.0	40.4
psbA_p4	TCCTTCAACGATTCATATACACT	ATACGTGTGCTTGGGAGTTC	34.8	50.0	55.2	57.9	1146 bp	1.0	40.3
psbA_p5	TCCTTCAACGATTCATATACACT	AATACGTGTGCTTGGGAGTT	34.8	45.0	55.2	57.4	1147 bp	1.0	40.3