Hotspot

ndhD

SSC · 117958-119460 · 1503 bp

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Pi 0.00174

MarkerSeek score 37.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.00174
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GACTTTTTGTCTCATTAATGAAACA	TTTCGAGCACGGGTTTTTCT	28.0	45.0	54.8	58.3	1573 bp	1.0	40.4
ndhD_p2	GACTTTTTGTCTCATTAATGAAACA	GACAAAAGCCCGTACTCGAT	28.0	50.0	54.8	58.0	1604 bp	1.0	40.3
ndhD_p3	ACTTTTTGTCTCATTAATGAAACA	GACAAAAGCCCGTACTCGAT	25.0	50.0	53.7	58.0	1603 bp	1.0	40.3
ndhD_p4	GACTTTTTGTCTCATTAATGAAACA	TTACCGACAAAAGCCCGTAC	28.0	50.0	54.8	58.2	1609 bp	1.0	40.3
ndhD_p5	ACTTTTTGTCTCATTAATGAAACA	TTACCGACAAAAGCCCGTAC	25.0	50.0	53.7	58.2	1608 bp	1.0	40.3