Hotspot

matK

LSC · 1734-3278 · 1545 bp

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Pi 0.00396

MarkerSeek score 50.40

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1545
variable_sites: 22
pi: 0.00396
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCTCATCACGTCAACAAACCA	ATGAAATAGGTCCCGCCTCT	42.9	50.0	57.8	58.2	1606 bp	1.0	70.1
matK_p2	GTCTCATCACGTCAACAAACC	ATGAAATAGGTCCCGCCTCT	47.6	50.0	57.5	58.2	1607 bp	1.0	67.4
matK_p3	TCTCATCACGTCAACAAACCA	AATGAAATAGGTCCCGCCTC	42.9	50.0	57.8	57.4	1607 bp	1.0	66.5
matK_p4	CTCATCACGTCAACAAACCA	ATGAAATAGGTCCCGCCTCT	45.0	50.0	56.3	58.2	1605 bp	1.0	65.9
matK_p5	TCTCATCACGTCAACAAACCA	CGCCTCTGGTTCAAGTAGAA	42.9	50.0	57.8	57.3	1593 bp	1.0	65.7