Hotspot

petA-psbJ

LSC · 64343-65351 · 1009 bp

Back to Phoenix

Pi 0.015

MarkerSeek score 46.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 30
pi: 0.015
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCCAATGTAGGAGGG	GTTGATAACCGGCCGTTTTG	55.0	50.0	58.0	58.0	1760-1766 bp	1.0	81.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	GTTGATAACCGGCCGTTTTG	50.0	50.0	58.0	58.0	1706-1712 bp	1.0	81.0
petA-psbJ_p3	GCAATCCCAATGTAGGAGGG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1867-1873 bp	1.0	80.9
petA-psbJ_p4	GCAATCCCAATGTAGGAGGG	GGGTTTAGCTTACGACGTGT	55.0	50.0	58.0	57.9	1832-1838 bp	1.0	80.8
petA-psbJ_p5	TAGGTGGGAACAGAGGAAGG	GTTGATAACCGGCCGTTTTG	55.0	50.0	58.0	58.0	1997-2003 bp	1.0	80.7