Hotspot

accD-psaI

LSC · 60619-61235 · 617 bp

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Pi 0.00723

MarkerSeek score 63.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 14
pi: 0.00723
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	726-1020 bp	1.0	82.7
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	727-1021 bp	1.0	82.7
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	732-1026 bp	1.0	82.1
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	733-1027 bp	1.0	82.1
accD-psaI_p5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	732-1026 bp	1.0	81.9