Hotspot

trnG-GCC-trnfM-CAU

LSC · 37201-37411 · 211 bp

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Pi 0.00579

MarkerSeek score 51.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 4
pi: 0.00579
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CATATGCGTGCGTATCAGGA	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	335-383 bp	1.0	84.1
trnG-GCC-trnfM-CAU_p2	CATATGCGTGCGTATCAGGA	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	329-377 bp	1.0	83.5
trnG-GCC-trnfM-CAU_p3	TATGCGTGCGTATCAGGAAC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.2	58.0	333-381 bp	1.0	83.4
trnG-GCC-trnfM-CAU_p4	CATATGCGTGCGTATCAGGA	TTTCGTCCATTGGTTCCCAG	50.0	50.0	57.9	58.1	403-451 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p5	AGGAACGAAGAAAATGCGGA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.8	58.0	319-367 bp	1.0	83.2