Hotspot

trnS-UGA-psbZ

LSC · 36301-36652 · 352 bp

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Pi 0.00221

MarkerSeek score 29.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 2
pi: 0.00221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	GCAAAAACAGCTAATTGGAAAGC	55.0	39.1	58.1	58.0	420-433 bp	1.0	57.3
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	GCAAAAACAGCTAATTGGAAAGC	55.0	39.1	58.1	58.0	409-422 bp	1.0	56.7
trnS-UGA-psbZ_p3	AAGACCGGAGCTATCAACCA	GCAAAAACAGCTAATTGGAAAGC	50.0	39.1	58.4	58.0	419-432 bp	1.0	54.3
trnS-UGA-psbZ_p4	GGTTTTCAAGACCGGAGCTA	GCAAAAACAGCTAATTGGAAAGC	50.0	39.1	57.5	58.0	426-439 bp	1.0	54.0
trnS-UGA-psbZ_p5	TATCAACCACTCGGCCATCT	GCAAAAACAGCTAATTGGAAAGC	50.0	39.1	58.5	58.0	408-421 bp	1.0	53.7