Hotspot

trnT-GGU-psbD

LSC · 32579-33639 · 1061 bp

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Pi 0.00157
MarkerSeek score 57.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1061
variable_sites
6
pi
0.00157
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1217-1226 bp 1.0 78.3
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA CCCTCCGTAACCAATCATCC 50.0 55.0 57.9 57.7 1201-1210 bp 1.0 78.3
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA GCCAGACCAGCCTACAAAAA 50.0 50.0 57.9 58.4 1230-1239 bp 1.0 77.2
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 50.0 50.0 57.9 57.6 1216-1225 bp 1.0 77.1
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.3 58.2 1180-1189 bp 1.0 76.9