Hotspot

trnK-UUU-rps16

LSC · 4125-4867 · 743 bp

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Pi 0.00589
MarkerSeek score 58.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
743
variable_sites
10
pi
0.00589
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATCTGTTGC GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 891-920 bp 1.0 73.5
trnK-UUU-rps16_p2 GTCTGGACGAATCTGTTGCT GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 890-919 bp 1.0 73.5
trnK-UUU-rps16_p3 AGTCTGGACGAATCTGTTGC GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 893-922 bp 1.0 73.3
trnK-UUU-rps16_p4 GTCTGGACGAATCTGTTGCT GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 892-921 bp 1.0 73.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 834-863 bp 1.0 73.1