Hotspot

ycf1

Genome · 128575-134139 · 5565 bp

Pi 0.00301

MarkerSeek score 45.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5565
variable_sites: 70
pi: 0.00301
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTCTCTACGACGCCTAGGG	TCGAACCAGTACGTCTGTCT	55.0	50.0	58.4	58.1	246 bp	1.0	77.3
ycf1_p2	ATTCTCTACGACGCCTAGGG	TCTCGAACCAGTACGTCTGT	55.0	50.0	58.4	58.1	248 bp	1.0	77.3
ycf1_p3	GCCCCCAGTTCCTTACATTG	TCGAACCAGTACGTCTGTCT	55.0	50.0	58.5	58.1	339 bp	1.0	76.3
ycf1_p4	GCCCCCAGTTCCTTACATTG	TCTCGAACCAGTACGTCTGT	55.0	50.0	58.5	58.1	341 bp	1.0	76.3
ycf1_p5	ATTCTCTACGACGCCTAGGG	CTCGAACCAGTACGTCTGTC	55.0	55.0	58.4	57.8	247 bp	1.0	76.2