Hotspot

atpF

Genome · 13045-14326 · 1282 bp

Back to Phoebe

Pi 0.00153
MarkerSeek score 51.40
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
Genome
length_bp
1282
variable_sites
18
pi
0.00153
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACTAATTTCGTCGGCTCGAA GGGGGCGAAGTGATACAAAA 45.0 50.0 57.4 58.2 1438-1448 bp 1.0 75.3
atpF_p2 ACTAATTTCGTCGGCTCGAA CAATCCAAAACAAAGGGGGC 45.0 50.0 57.4 57.8 1452-1462 bp 1.0 75.2
atpF_p3 AATTTCGTCGGCTCGAATGG GGGGGCGAAGTGATACAAAA 50.0 50.0 59.0 58.2 1435-1445 bp 1.0 72.5
atpF_p4 AATTTCGTCGGCTCGAATGG CAATCCAAAACAAAGGGGGC 50.0 50.0 59.0 57.8 1449-1459 bp 1.0 72.3
atpF_p5 ACTAATTTCGTCGGCTCGAA CAAAGGGGGCGAAGTGATAC 45.0 55.0 57.4 58.6 1442-1452 bp 1.0 71.7