Hotspot

atpA

Genome · 11453-12976 · 1524 bp

Pi 0.00187

MarkerSeek score 31.30

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1524
variable_sites: 30
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	TAATCAGGTGCGACAACGAG	50.0	50.0	58.0	58.0	2083-2089 bp	1.0	81.4
atpA_p2	GTGCATTCAGACGAAAAGGC	TAATCAGGTGCGACAACGAG	50.0	50.0	58.0	58.0	2320-2325 bp	1.0	81.4
atpA_p3	TCAGCTCGGTGGAACAAAAA	TAATCAGGTGCGACAACGAG	45.0	50.0	58.0	58.0	2452-2457 bp	1.0	81.3
atpA_p4	ATCGGGACCATTTCTCAACG	TAATCAGGTGCGACAACGAG	50.0	50.0	58.0	58.0	1786-1790 bp	0.952381	78.1
atpA_p5	CGGGACCATTTCTCAACGAT	TAATCAGGTGCGACAACGAG	50.0	50.0	58.0	58.0	1784-1788 bp	0.857143	74.3