Hotspot

ccsA-ndhD

Genome · 120003-120243 · 241 bp

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Pi 0.0117
MarkerSeek score 42.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
Genome
length_bp
241
variable_sites
7
pi
0.0117
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTGGATTCGGCAATTGTGG TCCTGATTTCGTTCTCTCGC 52.6 50.0 57.3 57.7 429-431 bp 1.0 66.8
ccsA-ndhD_p2 GTGGATTCGGCAATTGTGG CCTGATTTCGTTCTCTCGCT 52.6 50.0 57.3 57.7 428-430 bp 1.0 66.7
ccsA-ndhD_p3 TGGATTCGGCAATTGTGGC TCCTGATTTCGTTCTCTCGC 52.6 50.0 59.1 57.7 428-430 bp 1.0 63.7
ccsA-ndhD_p4 TGGATTCGGCAATTGTGGC CCTGATTTCGTTCTCTCGCT 52.6 50.0 59.1 57.7 427-429 bp 1.0 63.6
ccsA-ndhD_p5 GGCAATTGTGGCTTCTATAGGA TCCTGATTTCGTTCTCTCGC 45.5 50.0 58.2 57.7 421-423 bp 1.0 63.2