Hotspot

rpl32-trnL-UAG

Genome · 117496-118884 · 1389 bp

Back to Phoebe

Pi 0.00455
MarkerSeek score 55.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
1389
variable_sites
33
pi
0.00455
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAGGGGATATCAGGCC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.5 57.9 1528-1543 bp 1.0 77.6
rpl32-trnL-UAG_p2 AAGGAAGGGGATATCAGGCC AGCAGCGTGTCTACCAATTT 55.0 45.0 58.5 57.8 1521-1536 bp 1.0 77.1
rpl32-trnL-UAG_p3 AAGGAAGGGGATATCAGGCC CCTAAGAGCAGCGTGTCTAC 55.0 55.0 58.5 57.8 1527-1542 bp 1.0 77.0
rpl32-trnL-UAG_p4 AAGGAAGGGGATATCAGGCC CTAAGAGCAGCGTGTCTACC 55.0 55.0 58.5 57.8 1526-1541 bp 1.0 77.0
rpl32-trnL-UAG_p5 GCGGCAAAAGCTTTATCCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.3 57.9 1521 bp 0.047619 35.7