Hotspot

ndhF-rpl32

Genome · 116087-117321 · 1235 bp

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Pi 0.0051
MarkerSeek score 65.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
Genome
length_bp
1235
variable_sites
29
pi
0.0051
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACAAGCAGAAGTCCCAA GTACGTTTCTTTGGAACCGC 50.0 50.0 58.0 57.7 1261-1373 bp 1.0 83.1
ndhF-rpl32_p2 AGCAGAAGTCCCAACCCTAT GTACGTTTCTTTGGAACCGC 50.0 50.0 58.0 57.7 1255-1367 bp 1.0 83.1
ndhF-rpl32_p3 AAGCAGAAGTCCCAACCCTA GTACGTTTCTTTGGAACCGC 50.0 50.0 58.3 57.7 1256-1368 bp 1.0 81.2
ndhF-rpl32_p4 GGAACAAGCAGAAGTCCCAA GGCCTGATATCCCCTTCCTT 50.0 55.0 58.0 58.5 1319-1431 bp 1.0 81.2
ndhF-rpl32_p5 AGCAGAAGTCCCAACCCTAT GGCCTGATATCCCCTTCCTT 50.0 55.0 58.0 58.5 1313-1425 bp 1.0 81.1