Hotspot

rpl2-rpl23

Genome · 89398-89430 · 33 bp

Back to Phoebe

Pi 0.0364
MarkerSeek score 68.30
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
Genome
length_bp
33
variable_sites
3
pi
0.0364
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 TCGCGGATTGGATTTCACTT CGCTTCAACCGGGTTATTCT 45.0 50.0 57.9 58.3 170-175 bp 1.0 79.8
rpl2-rpl23_p2 TTCGCGGATTGGATTTCACT CGCTTCAACCGGGTTATTCT 45.0 50.0 57.9 58.3 171-176 bp 1.0 79.8
rpl2-rpl23_p3 CGCGGATTGGATTTCACTTG CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 169-174 bp 1.0 79.2
rpl2-rpl23_p4 TCGCGGATTGGATTTCACTT ATCATTACGCTTCAACCGGG 45.0 50.0 57.9 58.3 177-182 bp 1.0 79.1
rpl2-rpl23_p5 TTCGCGGATTGGATTTCACT ATCATTACGCTTCAACCGGG 45.0 50.0 57.9 58.3 178-183 bp 1.0 79.1