Hotspot

petA-psbJ

Genome · 66411-67562 · 1152 bp

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Pi 0.0111
MarkerSeek score 64.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
1152
variable_sites
41
pi
0.0111
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTCCAAGGTTTGTTGT GGCTCATATTCCGGATTGGG 45.0 55.0 58.2 58.2 1293-1372 bp 1.0 80.2
petA-psbJ_p2 ACGTGTCCAAGGTTTGTTGT CGGCTCATATTCCGGATTGG 45.0 55.0 58.2 58.6 1294-1373 bp 1.0 77.1
petA-psbJ_p3 ACGTGTCCAAGGTTTGTTGT GCTCATATTCCGGATTGGGA 45.0 50.0 58.2 57.1 1292-1371 bp 1.0 74.7
petA-psbJ_p4 ACGTGTCCAAGGTTTGTTGT ATTCCGGATTGGGATCATCC 45.0 50.0 58.2 57.1 1286-1365 bp 1.0 74.5
petA-psbJ_p5 CGTGTCCAAGGTTTGTTGTT GGCTCATATTCCGGATTGGG 45.0 55.0 57.1 58.2 1292-1371 bp 1.0 74.1