Hotspot

trnF-GAA-ndhJ

Genome · 50425-50991 · 567 bp

Back to Phoebe

Pi 0.00297
MarkerSeek score 54.40
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
Genome
length_bp
567
variable_sites
9
pi
0.00297
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 701-777 bp 1.0 84.5
trnF-GAA-ndhJ_p2 TCCTCTAATAGGGCGATGCA ATCCACGCCTGAAACGTATC 50.0 50.0 58.0 58.1 733-809 bp 1.0 84.5
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 665-741 bp 1.0 84.1
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 689-765 bp 1.0 83.4
trnF-GAA-ndhJ_p5 TGACATAGACCCCAGTCCTC ATCCACGCCTGAAACGTATC 55.0 50.0 57.8 58.1 748-824 bp 0.952381 81.2