Hotspot

trnH-GUG-psbA

Genome · 78-471 · 394 bp

Back to Phoebe

Pi 0.00996
MarkerSeek score 61.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
Genome
length_bp
394
variable_sites
21
pi
0.00996
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 452-473 bp 1.0 86.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 466-487 bp 1.0 85.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 465-486 bp 1.0 85.8
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 467-488 bp 1.0 85.8
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.5 57.9 481-502 bp 1.0 83.0