Hotspot

rps15

SSC · 121079-121414 · 336 bp

Back to Phlomoides

Pi 0.012

MarkerSeek score 44.70

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 336
variable_sites: 32
pi: 0.012
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GATCAGCCGAAGAACACCAT	TACCAAGAGCAAGGACATGC	50.0	50.0	58.0	57.9	1193-1236 bp	0.957447	85.7
rps15_p2	AACACCATGCATTGATGGGT	TACCAAGAGCAAGGACATGC	45.0	50.0	58.1	57.9	1181-1224 bp	0.93617	84.4
rps15_p3	CTTCTCCGCCAATAATGCCT	TACCAAGAGCAAGGACATGC	50.0	50.0	58.0	57.9	1743-1786 bp	0.957447	84.3
rps15_p4	TCTTCTCCGCCAATAATGCC	TACCAAGAGCAAGGACATGC	50.0	50.0	58.0	57.9	1744-1787 bp	0.957447	84.3
rps15_p5	CTCAAACGGTTTCACGCTTC	TACCAAGAGCAAGGACATGC	50.0	50.0	58.0	57.9	1034-1048 bp	0.382979	59.3