Hotspot

ndhF

SSC · 108752-110974 · 2223 bp

Back to Phlomoides

Pi 0.0111

MarkerSeek score 55.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 187
pi: 0.0111
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2519-2524 bp	0.93617	83.2
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2518-2523 bp	0.93617	83.2
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2511-2516 bp	0.93617	83.2
ndhF_p4	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2510-2515 bp	0.93617	83.2
ndhF_p5	GGGAGAAAGGGTGGAAAGTG	AAGAGGTAAGGTCGGGTGAA	55.0	50.0	58.1	58.0	2590-2595 bp	0.93617	82.7