Hotspot

ccsA

SSC · 112347-113318 · 972 bp

Back to Phlomoides

Pi 0.00976

MarkerSeek score 49.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 69
pi: 0.00976
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1677-1686 bp	1.0	86.2
ccsA_p2	TGAAATCGGTAGACACGCTG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1676-1685 bp	1.0	86.2
ccsA_p3	GTGAAATCGGTAGACACGCT	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1676-1685 bp	1.0	86.2
ccsA_p4	TGAAATCGGTAGACACGCTG	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1675-1684 bp	1.0	86.2
ccsA_p5	GTGAAATCGGTAGACACGCT	TGACGAAATGGGCGGAATAG	50.0	50.0	58.0	58.1	1727-1736 bp	1.0	85.9