Hotspot

rpl32-trnL-UAG

SSC · 111657-112182 · 526 bp

Back to Phlomoides

Pi 0.0172
MarkerSeek score 64.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
526
variable_sites
71
pi
0.0172
unique_haplotype_count
35
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAACT AGCGTGTCTACCGATTTCAC 33.3 50.0 54.9 58.0 484-610 bp 1.0 59.2
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAACT CAGCGTGTCTACCGATTTCA 33.3 50.0 54.9 58.0 485-611 bp 1.0 59.2
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAACT TCCTAAGAGCAGCGTGTCTA 33.3 50.0 54.9 57.9 494-620 bp 1.0 58.3
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAACT CCTAAGAGCAGCGTGTCTAC 33.3 55.0 54.9 57.8 493-619 bp 1.0 57.7
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAACT CTAAGAGCAGCGTGTCTACC 33.3 55.0 54.9 57.8 492-618 bp 1.0 57.7