Hotspot

trnT-UGU-trnL-UAA

LSC · 46071-46733 · 663 bp

Back to Phlomoides

Pi 0.0166

MarkerSeek score 62.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 66
pi: 0.0166
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGTTAGTCCTGGTAAAGCCC	GGATCAGGATCGCCCATTTT	55.0	50.0	58.0	58.0	1273-1361 bp	1.0	90.1
trnT-UGU-trnL-UAA_p2	TAGTCCTGGTAAAGCCCCAA	GGATCAGGATCGCCCATTTT	50.0	50.0	58.0	58.0	1270-1358 bp	1.0	89.9
trnT-UGU-trnL-UAA_p3	TTAGTCCTGGTAAAGCCCCA	GGATCAGGATCGCCCATTTT	50.0	50.0	58.0	58.0	1271-1359 bp	1.0	89.9
trnT-UGU-trnL-UAA_p4	CGTTAGTCCTGGTAAAGCCC	ACGCAGTCAACTCCATTTGT	55.0	45.0	58.0	58.0	1370-1458 bp	1.0	89.7
trnT-UGU-trnL-UAA_p5	CGTTAGTCCTGGTAAAGCCC	GGATTCCTCTACCAACGCAG	55.0	55.0	58.0	58.1	1384-1472 bp	0.978723	88.6