Hotspot

psaA-ycf3

LSC · 41635-42361 · 727 bp

Back to Phlomoides

Pi 0.00852

MarkerSeek score 67.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 48
pi: 0.00852
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	777-845 bp	1.0	83.0
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	781-849 bp	1.0	82.8
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.5	58.3	776-844 bp	1.0	82.0
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.5	57.6	770-838 bp	1.0	81.6
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.5	57.7	855-923 bp	1.0	81.4