Hotspot

rps16-trnQ-UUG

LSC · 6238-7230 · 993 bp

Back to Phlomoides

Pi 0.0134

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 102
pi: 0.0134
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	817-1150 bp	1.0	90.8
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	TGACTGGCATAACATCCACG	50.0	50.0	57.9	58.0	1276-1603 bp	1.0	90.2
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	AGCAGCTTGCCAAACAAAAG	50.0	45.0	57.9	58.1	1320-1647 bp	1.0	89.8
rps16-trnQ-UUG_p4	AACGGATCGTGTCCTTCAAG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	1325-1652 bp	1.0	89.7
rps16-trnQ-UUG_p5	AACGGATCGTGTCCTTCAAG	CGTGTGAATCGAGGGTTCAT	50.0	50.0	57.9	58.0	1442-1770 bp	0.957447	88.0