Hotspot

ycf1

SSC · 147174-151639 · 4466 bp

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Pi 0.00134

MarkerSeek score 54.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4466
variable_sites: 6
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGATTCGTAGATCCCGATTCT	ACGGACTCGATTGTGGAATG	42.9	50.0	55.8	58.0	312 bp	1.0	54.6
ycf1_p2	AGATTCGTAGATCCCGATTCT	CACGGACTCGATTGTGGAAT	42.9	50.0	55.8	58.0	313 bp	1.0	54.6
ycf1_p3	AGATTCGTAGATCCCGATTCT	CTCGAAACACGGACTCGATT	42.9	50.0	55.8	58.0	320 bp	1.0	54.5
ycf1_p4	AGATTCGTAGATCCCGATTCTT	ACGGACTCGATTGTGGAATG	40.9	50.0	56.5	58.0	312 bp	1.0	52.6
ycf1_p5	AGATTCGTAGATCCCGATTCTT	CACGGACTCGATTGTGGAAT	40.9	50.0	56.5	58.0	313 bp	1.0	52.6