Hotspot

psbH

LSC · 99156-99377 · 222 bp

Back to Phlomis

Pi 0.00901

MarkerSeek score 57.70

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 2
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TTTCAAGACCCCAACGGATC	AGCCAGGACTTTGTGATTCG	50.0	50.0	57.8	58.2	339 bp	1.0	77.4
psbH_p2	TCAAGACCCCAACGGATCTA	AGCCAGGACTTTGTGATTCG	50.0	50.0	57.8	58.2	337 bp	1.0	77.1
psbH_p3	AGACCCCAACGGATCTATCA	AGCCAGGACTTTGTGATTCG	50.0	50.0	57.5	58.2	334 bp	1.0	75.2
psbH_p4	TTTCAAGACCCCAACGGATC	GCCAGGACTTTGTGATTCGT	50.0	50.0	57.8	58.5	338 bp	1.0	75.1
psbH_p5	TCAAGACCCCAACGGATCTA	GCCAGGACTTTGTGATTCGT	50.0	50.0	57.8	58.5	336 bp	1.0	74.7