Hotspot

psaC

SSC · 140515-140760 · 246 bp

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Pi 0

MarkerSeek score 18.80

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 18.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCAAGGAAAATGGTTCGTGG	AGACCGAAAATTGATGGCGT	50.0	45.0	57.9	58.2	417-422 bp	1.0	79.6
psaC_p2	TTTGCCCACTGTACATTGCT	AGACCGAAAATTGATGGCGT	45.0	45.0	58.0	58.2	775-780 bp	1.0	79.4
psaC_p3	TTTGCCCACTGTACATTGCT	TGAAGCAGCTATTGGATCGG	45.0	50.0	58.0	57.8	1108-1113 bp	1.0	78.7
psaC_p4	GAGAATCTCGAGTAACCGGC	AGACCGAAAATTGATGGCGT	55.0	45.0	57.9	58.2	733-738 bp	1.0	78.6
psaC_p5	GCAAGGAAAATGGTTCGTGG	TGAAGCAGCTATTGGATCGG	50.0	50.0	57.9	57.8	750-755 bp	1.0	78.5