Hotspot

ndhA

SSC · 143076-145235 · 2160 bp

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Pi 0.00185

MarkerSeek score 58.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2160
variable_sites: 4
pi: 0.00185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGCATAGGGAACATATCGGA	ACTTGCAAATTCTTCCGCAG	45.0	45.0	55.5	57.3	2362-2363 bp	1.0	54.3
ndhA_p2	AGCATAGGGAACATATCGGA	CTTGCAAATTCTTCCGCAGT	45.0	45.0	55.5	57.3	2361-2362 bp	1.0	54.3
ndhA_p3	AGCATAGGGAACATATCGGAA	ACTTGCAAATTCTTCCGCAG	42.9	45.0	56.3	57.3	2362-2363 bp	1.0	52.8
ndhA_p4	AGCATAGGGAACATATCGGAA	CTTGCAAATTCTTCCGCAGT	42.9	45.0	56.3	57.3	2361-2362 bp	1.0	52.8
ndhA_p5	AGCATAGGGAACATATCGGA	TCAACTTGCAAATTCTTCCGC	45.0	42.9	55.5	58.0	2365-2366 bp	1.0	51.9