Hotspot

psbE-petL

LSC · 89821-90744 · 924 bp

Back to Phlomis

Pi 0.00216

MarkerSeek score 60.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 2
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1116 bp	1.0	72.2
psbE-petL_p2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1129 bp	1.0	71.9
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1039 bp	1.0	71.4
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1125 bp	1.0	71.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1124 bp	1.0	71.1