Hotspot

psbM-trnD-GUC

LSC · 55402-55930 · 529 bp

Back to Phlomis

Pi 0.00567

MarkerSeek score 54.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 3
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TAAGCAGAAAGGCGGTAGGA	TGCGAGATCCCCTATTTCCT	50.0	50.0	58.4	57.9	701 bp	1.0	76.0
psbM-trnD-GUC_p2	TAAGCAGAAAGGCGGTAGGA	ATTTCCTTTCCCCGGGATTG	50.0	50.0	58.4	57.8	688 bp	1.0	75.6
psbM-trnD-GUC_p3	ATAAGCAGAAAGGCGGTAGG	TGCGAGATCCCCTATTTCCT	50.0	50.0	57.4	57.9	702 bp	1.0	74.5
psbM-trnD-GUC_p4	TAAGCAGAAAGGCGGTAGGA	TTCCTTTCCCCGGGATTGTA	50.0	50.0	58.4	58.3	686 bp	1.0	74.2
psbM-trnD-GUC_p5	TAAGCAGAAAGGCGGTAGGA	TCCCCGGGATTGTAGTTCAA	50.0	50.0	58.4	58.3	680 bp	1.0	74.1