Hotspot

petN-psbM

LSC · 54500-55296 · 797 bp

Back to Phlomis

Pi 0.00502

MarkerSeek score 60.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 4
pi: 0.00502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	883 bp	1.0	76.8
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	881 bp	1.0	76.8
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	55.0	50.0	57.9	58.4	880 bp	1.0	76.2
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	882 bp	1.0	75.3
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	880 bp	1.0	75.3