Hotspot

rpoB-trnC-GCA

LSC · 52363-53501 · 1139 bp

Back to Phlomis

Pi 0.00176

MarkerSeek score 59.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 2
pi: 0.00176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1228 bp	1.0	77.6
rpoB-trnC-GCA_p2	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1304 bp	1.0	77.5
rpoB-trnC-GCA_p3	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1305 bp	1.0	77.4
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGGTAATCAGGCGACACC	50.0	50.0	57.8	58.2	1247 bp	1.0	76.8
rpoB-trnC-GCA_p5	GTTCTTCTGTCAAACCCCGA	TTTGGTAATCAGGCGACACC	50.0	50.0	57.8	58.2	1323 bp	1.0	76.7