Hotspot

rpoB

LSC · 19041-22271 · 3231 bp

Pi 0.000877

MarkerSeek score 55.90

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3231
variable_sites: 5
pi: 0.000877
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ACCTCTTTTCACATCCGGAA	ATGGCCCGTAATAATCCAGC	45.0	50.0	56.8	57.8	239 bp	1.0	69.1
rpoB_p2	ACCTCTTTTCACATCCGGAA	CCGCGATATACGACGATTCA	45.0	50.0	56.8	57.8	217 bp	1.0	68.9
rpoB_p3	ACCTCTTTTCACATCCGGAA	GGCCCGTAATAATCCAGCTT	45.0	50.0	56.8	57.7	237 bp	1.0	68.5
rpoB_p4	ACCTCTTTTCACATCCGGAA	AAGTGGATACCTCAAGGCCA	45.0	50.0	56.8	58.3	262 bp	1.0	68.0
rpoB_p5	ACCTCTTTTCACATCCGGAA	AGTGGATACCTCAAGGCCAA	45.0	50.0	56.8	58.3	261 bp	1.0	68.0