Hotspot

petD

LSC · 72454-73674 · 1221 bp

Pi 0.00246

MarkerSeek score 51.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1221
variable_sites: 5
pi: 0.00246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACACATGTCATTTGGATACTTCT	GCACGGTACTTCACAGTTGA	34.8	50.0	55.9	58.1	1353 bp	1.0	40.3
petD_p2	ACACATGTCATTTGGATACTTCT	TGCACGGTACTTCACAGTTG	34.8	50.0	55.9	58.4	1354 bp	1.0	40.3
petD_p3	AACACATGTCATTTGGATACTTCT	GCACGGTACTTCACAGTTGA	33.3	50.0	56.6	58.1	1354 bp	1.0	40.3
petD_p4	ACACATGTCATTTGGATACTTCTT	GCACGGTACTTCACAGTTGA	33.3	50.0	56.6	58.1	1353 bp	1.0	40.3
petD_p5	ACACATGTCATTTGGATACTTCT	ATGCACGGTACTTCACAGTT	34.8	45.0	55.9	57.5	1355 bp	1.0	40.3