Hotspot

ndhE

SSC · 109088-109393 · 306 bp

Pi 0.00381

MarkerSeek score 41.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GTGCGCCTTTGTTTAGAGGA	TCGGTTCAATTCTTGTCCTCA	50.0	42.9	58.5	57.2	423 bp	1.0	62.4
ndhE_p2	TGCGCCTTTGTTTAGAGGAT	TCGGTTCAATTCTTGTCCTCA	45.0	42.9	57.5	57.2	422 bp	1.0	62.4
ndhE_p3	TGTGCGCCTTTGTTTAGAGG	TCGGTTCAATTCTTGTCCTCA	50.0	42.9	58.8	57.2	424 bp	1.0	60.2
ndhE_p4	GTGCGCCTTTGTTTAGAGGA	ATCGGTTCAATTCTTGTCCTCA	50.0	40.9	58.5	57.7	424 bp	1.0	58.2
ndhE_p5	GTGCGCCTTTGTTTAGAGGA	TCGGTTCAATTCTTGTCCTCAT	50.0	40.9	58.5	57.7	423 bp	1.0	58.2