Hotspot

ndhA

SSC · 111065-113192 · 2128 bp

Pi 0.00102

MarkerSeek score 58.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2128
variable_sites: 4
pi: 0.00102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGACCGTTAAA	47.6	50.0	58.1	57.7	2267 bp	1.0	69.3
ndhA_p2	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGACCGTTA	47.6	50.0	58.1	58.6	2269 bp	1.0	66.4
ndhA_p3	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	47.6	50.0	58.1	58.6	2270 bp	1.0	66.4
ndhA_p4	ACCCAGTTACCATAGGGAACA	TTATGGGGGAAGTTGACCGT	47.6	50.0	58.1	58.6	2271 bp	1.0	66.4
ndhA_p5	ACCCAGTTACCATAGGGAACA	ATTATGGGGGAAGTTGACCG	47.6	50.0	58.1	57.3	2272 bp	1.0	65.7